Abnormal neuroinflammation ignited by overproduction of chemokines and cytokines via microglial cells can induce the occurrence and development of neurodegenerative disorders. The aim of this study is to investigate the effects of dexamethasone sodium phosphate (Dex-SP) on chemokine and cytokine secretion in lipopolysaccharide (LPS)-activated microglial cells. LPS markedly enhanced the secretion of pro-inflammatory factors such as regulated on activation, normal T cell expressed and secreted (RANTES), transforming growth factor beta-beta 1 (TGF-beta 1) and nitric oxide (NO), but decreased the production of macrophage inflammatory protein-1 alpha (MIP-1 alpha) and interleukin 10 (IL-10) in BV-2 microglial cells. Furthermore, LPS increased BV-2 microglial cell migration. However, Dex-SP treatment had the opposite effect, dampening the secretion of RANTES, TGF-beta 1, and NO, while increasing the production of MIP-1 alpha and IL-10 and blocking migration of LPS-stimulated BV-2 microglial cells. Furthermore, Dex-SP markedly suppressed the LPS-induced degradation of IRAK-1 and IRAK-4, and blocked the activation in TRAF6, p-TAK1, and p-JNK in BV-2 microglial cells. These results showed that Dex-SP inhibited the neuroinflammatory response and migration in LPS-activated BV-2 microglia by inhibiting the secretion of RANTES, TGF-beta 1, and NO and increasing the production of MIP-1 alpha and IL-10. The molecular mechanism of Dex-SP may be associated with inhibition of TRAF6/TAK-1/JNK signaling pathways mediated by IRAK-1 and IRAK-4.
[1]Shanghai Univ Med & Hlth Sci, Coll Pharm, Shanghai, Peoples R China.
[2]Shanghai Univ Med & Hlth Sci, Hlth Sch, Shanghai, Peoples R China.
[3]Jiyuan Shi Peoples Hosp, Jiyuan, Henan, Peoples R China.
[4]Shanghai Jiao Tong Univ, Dept Emergency Med, Peoples Hosp 6, Shanghai, Peoples R China.
[5]Jiaxing Univ, Coll Med, Jiaxing, Peoples R China.
(8.0+极速模式)