Lumican is a dermatan sulfate proteoglycan highly expressed in connective tissue and has the ability to regulate collagen fibril assembly. Previous studies have shown that lumican is involved in wound healing, but the precise effects of lumican on reepithelialization and wound contraction, the two pivotal aspects of skin wound healing, have not been investigated. Here we explored the roles of lumican in fibroblast contractility, a main aspect of skin wound healing, by adopting mice skin wound healing model and the corresponding in vitro cellular experiments. Our results showed that lumican can promote skin wound healing by facilitating wound fibroblast activation and contraction but not by promoting keratinocyte proliferation and migration. Silencing of integrin alpha 2 completely abolished the pro-contractility of lumican, indicating lumican enhances fibroblast contractility via integrin alpha 2. Our study for the first time demonstrated that lumican can affect fibroblast's mechanical property, which is pivotal for many important pathological processes, such as wound healing, fibrosis, and tumor development, suggesting that lumican might have a potential to be used to modulate these processes.
[1]Shanghai Jiao Tong Univ, Dept Plast Surg, Affiliated Peoples Hosp 6, Shanghai 200030, Peoples R China.
[2]Shanghai Jiao Tong Univ, Sch Med, State Key Lab Oncogenes & Related Genes, Shanghai Canc Inst,Ren Ji Hosp, Shanghai 200030, Peoples R China.
[3]Cent Hosp Fengxian Dist, Dept Gen Surg, Shanghai, Peoples R China.
(8.0+极速模式)