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Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase 期刊论文

编号：

0e1a7b1a-7333-4c72-88db-644137075dfd
作者：

Chen, LiFeng#^[1]Zhang, YiPing^[1];Fan, HaiYang^[1];Wu, Kai(吴锴)^[2]Lin, JinPing^[1];Wang, HuaLei*^[1]Wei, DongZhi^[1];
语种：

英文
期刊：

CATALYSIS COMMUNICATIONS ISSN：1566-7367 2017 年 97 卷 (5 - 9) ; JUL 5
收录：
关键词：

(R)-N-Boc-3-hydroxypiperidine Carbonyl reductase Asymmetric reduction Cofactor-recycling system
摘要：

The 3-hydroxypiperidine moiety is a privileged scaffold encountered in many bioactive compounds. An NADPH-dependent reductase (YGL039W) from Kluyveromyces marxianus ATCC 748 was isolated to show excellent catalytic activity in (R)-N-Boc-3-hydroxypiperidine [(R)-NBHP] production. Using a GDH-catalyzed cofactor recycling system to ensure a sufficient supply of NADPH, the effects of temperature, pH, metal ions, substrate concentration, biocatalyst dosage, and cofactors on the YGL039W-catalyzed bioreduction were investigated and optimized. Finally, an extremely high concentration of N-Boc-piperidin-3-one (NBPO, 400 g/L) could be completely reduced to (R)-NBHP (> 99% ee), with a total turnover number of 20,000. This process shows significant potential for the industrial production of (R)-NBHP.
推荐引用方式
GB/T 7714：

Chen Li-Feng,Zhang Yi-Ping,Fan Hai-Yang, et al. Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase [J].CATALYSIS COMMUNICATIONS,2017,97:5-9.
APA：

Chen Li-Feng,Zhang Yi-Ping,Fan Hai-Yang,Wu Kai,&Wei Dong-Zhi.(2017).Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase .CATALYSIS COMMUNICATIONS,97:5-9.
MLA：

Chen Li-Feng, et al. "Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase" .CATALYSIS COMMUNICATIONS 97(2017):5-9.
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