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N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus 期刊论文

编号：

166a7ed2-6385-4d8e-b3d3-ba3170e1f081
作者：

Li, Lingmeng#^[1]Dong, Fengying^[1];Lin, Lin^[2,3]He, Dannong^[3];Wei, Wei*^[1]Wei, Dongzhi*^[1]
语种：

英文
期刊：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ISSN：0021-8561 2018 年 66 卷 41 期 (10788 - 10798) ; OCT 17
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关键词：

Geobacillus thermocatenulatus pullulanase domain truncation and insertion maize starch
摘要：

A novel thermostable type I pullulanase gene (pul(GT)) from Geobacillus thermocatenulatus DSMZ730 was cloned. It has an open reading frame of 2154 bp encoding 718 amino acids. G. thermocatenulatus pullulanase (Pul(GT)) was found to be optimally active at pH 6.5 and 70 degrees C. It exhibited stable activity in the pH range of 5.5-7.0. Pul(GT) lacked three domains (CBM41 domain, X25 domain, and X45 domain) compared with the pullulanase from Bacillus acidopullulyticus (2WAN). Different N-terminally domain truncated (730T) or spliced (730T-U1 and 730T-U2) mutants were constructed. Truncating the N-terminal 85 amino acids decreased the K-m value and did not change its optimum pH, an advantageous biochemical property in some applications. Compared with 2WAN, Pul(GT) can be used directly for maize starch saccharification without adjusting the pH, which reduces cost and improves efficiency.
推荐引用方式
GB/T 7714：

Li Lingmeng,Dong Fengying,Lin Lin, et al. N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus [J].JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018,66(41):10788-10798.
APA：

Li Lingmeng,Dong Fengying,Lin Lin,He Dannong,&Wei Dongzhi.(2018).N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus .JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,66(41):10788-10798.
MLA：

Li Lingmeng, et al. "N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus" .JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66,41(2018):10788-10798.
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