Ulva prolifera can protect human skin fibroblast from being injured by hydrogen peroxide. This work studied the composition of Ulva prolifera polysaccharide and identified its physicochemical properties. The results showed that the cell proliferation of 0.5 mgimL crude polysaccharide was 154.4% of that in negative control group. Moreover, ROS detection indices, including DCFH-DA, GSH-PX, MDA and CAT, indicated that crude polysaccharide could improve cellular ability to scavenge free radical and decrease the injury on human skin fibroblast by hydrogen peroxide. In purified polysaccharide, the activity of fraction P1-1 was the highest, with 174.6% of that in negative control group. The average molecular weight of P1-1 was 137 kD with 18.0% of sulfate content. This work showed the inhibition of hydrogen peroxide induced injuries on human skin fibroblast by Ulva prolifera polysaccharide, which may further evaluate the application of U. prolifera on cosmetics. (C) 2016 Elsevier B.V. All rights reserved.
[1]Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China.
[2]Shanghai Ocean Univ, Inst Marine Sci, Shanghai 201306, Peoples R China.
[3]Second Mil Med Univ, Marine Biomed Inst, Shanghai 200433, Peoples R China.
[4]Shanghai Univ Med & Hlth Sci, Shanghai 201318, Peoples R China.
(8.0+极速模式)