With the advent of DNA nanotechnology, nucleic acids have been used building blocks for constructing various DNA nanostructures. As classical and simple DNA nanostructures, framework nucleic acids (FNAs) have attracted enormous attention in the field of biosensing. The sequence-specific self-assembly properties and high programmability of nucleic acids allow FNAs to be incorporated in advanced probe design. In addition, FNAs enable the engineering of surfaces for biosensing (e. g., probe orientation, probe spacing), thereby improving the accessibility of target molecules to the probes arranged on heterogeneous surfaces. Moreover, FNAs offer a universal and promising platform for sensing cellular molecules because of their prominent biocompatibility and cellular permeability. Herein recent advances in FNA-based biosensors are summarized, including electrochemical detection, optical detection, and intracellular sensing. It is hoped that this review will provide guidelines for the design and construction of FNA scaffold-based biosensors.
[1]East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, 500 Dongchuan Rd, Shanghai 200241, Peoples R China.
[2]Shanghai Jiao Tong Univ, Joint Res Ctr Precis Med, Shanghai 201499, Peoples R China.
[3]Affiliated Sixth Peoples Hosp South Campus, 6600th Nanfeng Rd, Shanghai 201499, Peoples R China.
[4]Southern Med Univ, Affiliated Fengxian Hosp, Shanghai 201499, Peoples R China.
(8.0+极速模式)