To study the value of 18F-FDG coincidence imaging in diagnosis of cancer, 101 patients who were suspicious of cancer were 18F-FDG coincidence imaged before operation. Diagnoses of visual and semi-quantitative analysis were compared with the pathologic results after operation. The sensitivity and speciality of 18F-FDG coincidence imaging were 84.61% and 78.26% in detecting cancer, while accuracy was 83.1%. For patients with a cancer of less than 2 cm, the sensitivity and speciality were 80% and 100%, accuracy was 82.35%. When standard of diagnosis was higher than 2.0 in T/N value, the sensitivity and speciality were 85.90% and 82.61%, while accuracy was 85.15%; when visual analysis was combined with semi-quantitative analysis, the sensitivity and speciality were 88.5% and 87.0%, while accuracy was 88.11%. In conclusion, it is of higher value to diagnose cancers with 18F-FDG coincidence imaging, and the accuracy of diagnosis can be improved by visual analysis combined with semi-quantitative analysis.