Purpose: The postmortem interval (PMI) is the amount of time that has elapsed since the time of death. Over the years, many approaches have been developed to assess PMI, but their time frame of applicability has been only days to weeks. Our present pilot study aimed to find the sensitive mRNA marker if the degradation of mRNA could be used to estimate the early postmortem interval (up to 24 h).
Methods: In our study, we use the microarray to screen 217 mRNAs markers of rat cardiac tissue. Then, real-time fluorescent quantitative PCR (qPCR) was used to validate of the candidate markers at 7 time points within 24 h and at temperatures of 25 degrees C and 35 degrees C. Another 27 rats were then used to verify the model.
Results: Among all of the candidate markers, Delta Cq (cell division cycle 25 homolog B(Cdc25b)) had the best correlation coefficient with early postmortem interval and was used to build a new model using the R software. The results of verification testing demonstrated that the error rate was less than 15%, demonstrating the high predictive power of our mathematical model.
Conclusion: In this study, Cdc25b was found to be the sensitive marker to estimate early postmortem interval, and Rpl27 was found to be suitable for use as the endogenous control. Our work provided new leads for molecular approaches to early postmortem interval estimation using the significant mRNA markers established here.
[1]Fudan Univ, Dept Forens Med, Sch Basic Med Sci, 131Dongan Rd, Shanghai 200032, Peoples R China.
[2]Shenzhen Inst Criminal Sci & Technol, Shenzhen Publ Secur Bur, Invest Dept, Key Lab Forens Pathol,Minist Publ Secur, Shenzhen 518000, Peoples R China.
[3]Fudan Univ, Childrens Hosp, 399 Wanyuan Rd, Shanghai 201102, Peoples R China.
[4]Shanghai Univ Med & Hlth Sci, 279 ZhouzhuHwy, Shanghai 201318, Peoples R China.
[5]Shanghai Publ Secur Bur, Criminal Sci & Technol Inst, Pudong Branch, Forens Lab, 255 Yanzhong Rd, Shanghai 200125, Peoples R China.
[6]Shanghai Publ Secur Bur, Criminal Sci & Technol Inst, Forens Lab, 803 North Zhongshan Rd, Shanghai 200082, Peoples R China.
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