Folate receptor targeted photo-luminescent quantum carbon dots (Fr-CDs) were successfully prepared from folic acid and phenylenediamine isomers through hydrothermal approaches. Fr-CDs were spherical particles smaller than 10 nm, and emit stable green, blue and red luminescence under ultraviolet region excitation (lambda(ex) = 365 nm) with maximum emissive lengths at 530, 429, and 612 nm. And the corresponding photoluminescence quantum yield as 15.4%, 12.6% and 16.2% respectively. Up-converted photoluminescent properties in near infrared 800 ran spectral region located in green, blue and yellow region. In-vitro studies showed Fr-CDs had almost none cytotoxicity (cell viability over 80%) and high affinitive to the Hela celline highly-expressed-folate-receptor membranes, and lighted on cytoplasm as the fluorescent marker. It displayed long luminescent-stability with PL intensity above 90% in ultraviolet illuminant exposure over 24 h. In in-vivo studies, Fr-CDs were internalized accumulated in targeted cancer tissues of cervical carcinoma and the emitting fluorescence maintains over 30 min.
[1]Natl Engn Res Ctr Nanotechnol, Shanghai 200241, Peoples R China.
[2]Shanghai Jiao Tong Univ, Sch Mat Sci & Engn, Shanghai 200240, Peoples R China.
[3]Shanghai Univ Med & Hlth Sci, Shanghai 200093, Peoples R China.
[4]Chonbuk Natl Univ, Adv Mat Inst BIN Convergence Technol, Dept BIN Convergence Technol, Jeonju 54896, South Korea.
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