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Alterations of DNA methylation profile in proximal jejunum potentially contribute to the beneficial effects of gastric bypass in a diabetic rat model 期刊论文

编号：

611a8ae9-4713-480b-9465-3e19f8931351
作者：

Liu, Haijun(刘海军)#^[1]Zhang, Hong#^[2]Wang, Xiao^[2];Yu, Xuemei(于雪梅)^[3]Hu, Cheng(胡承)*^[1,2]Zhang, Xueli(张学利)*^[1]
语种：

英文
期刊：

SURGERY FOR OBESITY AND RELATED DISEASES ISSN：1550-7289 2019 年 15 卷 8 期 (1291 - 1298) ; AUG
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关键词：

Type 2 diabetes Roux-en-Y gastric bypass DNA methylation Proximal jejunum
摘要：

Background: The foregut theory posits that the proximal small intestine plays an important role in the improvement of type 2 diabetes (T2D) after Roux-en-Y gastric bypass (RYGB).
Objective: To study the possible role of proximal jejunum in the treatment of T2D after RYGB via the analysis of DNA methylation and transcriptome.
Setting: Laboratory of Shanghai Diabetes Institute, China.
Methods: Two batches of T2D rats undergoing surgeries (RYGB and a sham operation as control) were established independently. The proximal jejuna from one batch were used for the methylated DNA immunoprecipitation sequencing and transcriptome sequencing. Those from another batch were used for DNA methylation analysis via a MassARRAY (Agena Bioscience, San Diego, CA, United States) platform and quantitative polymerase chain reaction to verify the results of high-throughput sequencing analysis.
Results: The transcriptome sequencing results showed that the genes differentially expressed (P < .05) in the RYGB- and sham-operated groups were significantly enriched in the gene ontology biological processes associated with fatty acid metabolism and lipid transport. The methylated DNA immunoprecipitation sequencing analysis showed that there were 1973 genes with significantly differentially methylated CpG sites located within gene promoters between the 2 groups (P < .05 and fold change > 2). The Mogat3 in the proximal jejunum after RYGB was hypermethylated in the promoter region and its transcription level was significantly reduced. The results of the high-throughput sequencing were validated via methylation quantitative analysis and quantitative polymerase chain reaction in a new set of samples.
Conclusions: RYGB could change DNA methylation and gene expression profiles in the proximal jejunum in T2D rats. Mogat3 potentially contributes to the beneficial effects caused by RYGB. (C) 2019 American Society for Bariatric Surgery. Published by Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Liu Haijun,Zhang Hong,Wang Xiao, et al. Alterations of DNA methylation profile in proximal jejunum potentially contribute to the beneficial effects of gastric bypass in a diabetic rat model [J].SURGERY FOR OBESITY AND RELATED DISEASES,2019,15(8):1291-1298.
APA：

Liu Haijun,Zhang Hong,Wang Xiao,Yu Xuemei,&Zhang Xueli.(2019).Alterations of DNA methylation profile in proximal jejunum potentially contribute to the beneficial effects of gastric bypass in a diabetic rat model .SURGERY FOR OBESITY AND RELATED DISEASES,15(8):1291-1298.
MLA：

Liu Haijun, et al. "Alterations of DNA methylation profile in proximal jejunum potentially contribute to the beneficial effects of gastric bypass in a diabetic rat model" .SURGERY FOR OBESITY AND RELATED DISEASES 15,8(2019):1291-1298.
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