In order to improve detection speed and accuracy of biological cells, a quantitative non-interference optical phase recovery method is proposed in commercial microscope, taking the red blood cells as the classical phase objects. Three bright field micrographs were collected in the experiment. Utilizing the transport intensity equation (TIE), the quantitative phase distributions of red blood cell are gained and agree well with the previous optical phase models. Analysis shows that the resolution of introduced system reaches sub-micron. This method not only quickly gives quantitative phase distribution of cells, but also measures a large number of cells simultaneously. So it is potential in the use of real-time observing and quantitative analyzing of cells in vivo.
[1]Shanghai Univ Elect Power, Coll Elect & Informat Engn, Shanghai 200090, Peoples R China.
[2]Shanghai Univ Med & Hlth Sci, Med Instrument Coll, Shanghai 201318, Peoples R China.
(8.0+极速模式)