Development of castration resistance is a key contributor to mortality in patients with prostate cancer. High expression of RING finger protein 7 (RNF7) in cancer cells is known to play a key role in tumor progression. However, the role of RNF7 in prostate cancer progression is not well elucidated. In this study, we silenced RNF7 by shRNA interference in two castration resistant prostate cancer (CRPC) cell lines, DU145 and PC3. RNF7 knockdown attenuated proliferation and enhanced sensitivity of prostate cancer cells to cisplatin treatment. Invasive property of DU145 and PC3 cells was also attenuated by RNF7 silencing. The underlying mechanisms appear to be associated with accumulation of tumor suppressive proteins p21, p27 and NOXA, while inactivation of ERK1/2 by RNF7 knockdown. We demonstrated that RNF7 knockdown induced growth suppression of prostate cancer cells and inactivated ERK1/2 pathway, which suggested RNF7 might be a potential novel therapeutic target for CRPC.
[1]Shanghai Univ Med & Hlth Sci, Shanghai Peoples Hosp East 6, Shanghai 201306, Peoples R China.
[2]East China Normal Univ, Joint Res Ctr Translat Med, Nanfeng Rd 6600, Shanghai 201499, Peoples R China.
[3]Southern Med Univ, Shanghai Fengxian Dist Cent Hosp, Nanfeng Rd 6600, Shanghai 201499, Peoples R China.
[4]East China Normal Univ, Shanghai Key Lab Regulatory Biol, Inst Biomed Sci, Shanghai 200241, Peoples R China.
[5]East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China.
[6]Tongji Univ, Shanghai Pulm Hosp, Dept Thorac Surg, Sch Med, Shanghai 200433, Peoples R China.
(8.0+极速模式)