Our previous study demonstrated that IL-10 secreting B (B10) cells alleviate inflammation and bone loss in experimental periodontitis. The purpose of this study is to determine whether antigen-specificity is required for the local infiltration of B10 cells. Experimental periodontitis was induced in the recipient mice by placement of silk ligature with or without the presence of live Porphyromonas gingivalis (P. gingivalis). Donor mice were preimmunized by intraperitoneal (IP) injection of formalin-fixed P. gingivalis, or PBS as non-immunized control. Spleen B cells were purified and treated with LPS and CpG for 48 h to expand the B10 population in vitro. Fluorescence-labelled B10 cells were transferred into the recipient mice by tail vein injection and were tracked on day 0, 3, 5 and 10 using IVIS Spectrum in vivo imaging system. The number of B10 cells and P. gingivalis-binding B cells were significantly increased after in vitro treatment of LPS and CpG. On day 5, the fluorescence intensity in gingival tissues was the highest in mice transferred with B10 cells from pre-immunized donor mice. Gingival expression of IL-6, TNF-alpha, RANKL/OPG ratio and periodontal bone loss in recipient mice were significantly reduced, and the expression of IL-10 and the number of CD19+ B cells were significantly increased after pre-immunized B10 cell transfer in the presence of antigen, compared to those with non-immunized B10 cell transfer or no antigen presence. This study suggests that antigen specificity dictate the local infiltration of B10 cells into periodontal tissue and these antigen-specific B10 cells promote anti-inflammatory responses.
[1]Shanghai Jiao Tong Univ, Peoples Hosp 9, Dept Oral Med, Shanghai Key Lab Stomatol,Sch Med, Shanghai 200011, Peoples R China.
[2]Natl Clin Res Ctr Stomatol, Shanghai Res Inst Stornatol, Shanghai 200011, Peoples R China.
[3]Harvard Sch Dent Med Affiliate, Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA 02142 USA.
[4]Harvard Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 USA.
[5]Qingdao Univ, Dept Stomatol, Affiliated Hosp, Qingdao 266003, Shandong, Peoples R China.
[6]Guangxi Med Univ, Affiliated Hosp Stomatol, Dept Prosthodont, Nanning 530021, Peoples R China.
[7]Shanghai Univ Med & Hlth Sci, Shanghai 201318, Peoples R China.
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