首页 / 院系成果 / 成果详情页

Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides  期刊论文  

  • 编号:
    9599193b-01f3-4d89-a7fe-e3993c0a72cb
  • 作者:
    Cai, Xianghai#[1]Wang, Wei*[1]Lin, Lin[2,3]He, Dannong[2,3]Huang, Gang(黄钢)[3]Shen, Yaling[1];Wei, Wei[1];Wei, Dongzhi[1];
  • 语种:
    英文
  • 期刊:
    SCIENTIFIC REPORTS ISSN:2045-2322 2017 年 7 卷 ; JUN 14
  • 收录:
  • 摘要:

    The EstPS1 gene, which encodes a novel carboxylesterase of Pseudomonas synxantha PS1 isolated from oil well-produced water, was cloned and sequenced. EstPS1 has an open reading frame of 1923 bp and encodes the 640-amino acid carboxylesterase (EstPS1), which contains an autotransporter (AT) domain (357-640 amino acids). Homology analysis revealed that EstPS1 shared the highest identity (88%) with EstA from Pseudomonas fluorescens A506 (NCBI database) and belonged to the carboxylesterase family (EC 3.1.1.1). The optimum pH and temperature of recombinant EstPS1 were found to be 8.0 and 60 degrees C, respectively. EstPS1 showed high thermostability, and the half-lives (T-1/2 thermal inactivation) at 60, 70, 80, 90, and 100 degrees C were 14 h, 2 h, 31 min, 10 min, and 1 min, respectively. To understand the role of the AT domain in carboxylesterase, AT domain-truncated carboxylesterase (EstPS1 Delta AT) was generated. EstPS1 Delta AT showed a clearly decreased secretion rate, owing to the AT domain strongly improved secretory expression in the heterogeneous system. EstPS1 degraded various pyrethroid pesticides, and hydrolysis efficiencies were dependent on the pyrethroid molecular structure. EstPS1 degraded all the tested pyrethroid pesticides and hydrolysed the p-nitrophenyl esters of medium-short-chain fatty acids, indicating that EstPS1 is an esterase with broad specificity.

  • 推荐引用方式
    GB/T 7714:
    Cai Xianghai,Wang Wei,Lin Lin, et al. Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides [J].SCIENTIFIC REPORTS,2017,7.
  • APA:
    Cai Xianghai,Wang Wei,Lin Lin,He Dannong,&Wei Dongzhi.(2017).Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides .SCIENTIFIC REPORTS,7.
  • MLA:
    Cai Xianghai, et al. "Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides" .SCIENTIFIC REPORTS 7(2017).
  • 条目包含文件:
    文件类型:PDF,文件大小:
    正在加载全文
浏览次数:39 下载次数:0
浏览次数:39
下载次数:0
打印次数:0
浏览器支持: Google Chrome   火狐   360浏览器极速模式(8.0+极速模式) 
返回顶部