Introduction: Breast cancer is the second leading cause of cancer death among females. We sought to identify microRNA (miRNA) markers in breast cancer, and determine whether miRNA expression is predictive of early stage breast cancer. The paired panel of microRNAs is promising.
Methods: Global miRNA expression profiling was performed on three pooling samples of plasma from breast cancer, benign lesion and normal, using next generation sequencing technology. Thirteen microRNAs (hsa-miR-21-3p, hsa-miR-192-5p, hsa-miR-221-3p, hsa-miR-451a, hsa-miR-574-5p, hsa-miR-1273g-3p, hsa-miR-152, hsa-miR-22-3p, hsa-miR-222-3p, hsa-miR-30a-5p, hsa-miR-30e-5p, hsa-miR-324-3p, and hsa-miR-382-5p) were subsequently validated using real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) in a cohort of 53 breast cancer, 40 benign lesions and 38 normal cases. The pairwise miRNA ratios were calculated as biomarkers to classify breast cancer.
Results: According to the model used to predict breast cancer from benign lesions, a panel of five miRNA pairs had high diagnostic power with an AUG of 0.942. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of this model after 10-fold cross validation were 0.881, 0.775, 0.827, and 0.756, respectively. In addition, the other panels of miRNA pairs distinguishing the breast cancer from normal and non-cancer patients had good performance.
Conclusion: Certain MicroRNA pairs were identified and deemed effective in breast cancer screening, especially when distinguishing cancer from benign lesions.
[1]Univ Hawaii Manoa, John A Burns Sch Med, Dept Complementary & Integrat Med, Bioinformat Core, Honolulu, HI 96822 USA.
[2]Shanghai Univ Med & Hlth Sci, Shanghai, Peoples R China.
[3]Nanjing Univ Chinese Med, Affiliated Hosp 3, Natl Med Ctr Colorectal Dis, Nanjing, Jiangsu, Peoples R China.
[4]Wuhan Univ Sci & Technol, Tianyou Hosp, Dept Anesthesiol, Wuhan, Hubei, Peoples R China.
[5]Presence Hlth, Des Plaines, IL USA.
[6]Rush Univ, Med Ctr, Dept Anat & Cell Biol, Chicago, IL 60612 USA.
[7]Fudan Univ, Sch Pharm, Dept Microbiol & Biochem Pharm, Shanghai, Peoples R China.
(8.0+极速模式)