Background: Radiation therapy is widely used for the treatment of pituitary adenomas. Unfortunately, it might raise the risk of ischemic stroke, with neuroinflammation being a major pathological process. Astrocytes are the most abundant cell type in the central nervous system and have been reported for playing important roles in ischemic stroke.
Objective: Here we studied how gamma-radiation would introduce astrocytes into a detrimental state for neuroinflammation and provide new theory evidence and target for the clinical management of inflammation-related neural damage after radiation-induced ischemic stroke.
Method: HA-1800 cells were treated with gamma-radiation and then the protein and mRNA levels of Connexin (Cx)-43 were evaluated by western and q-PCR. The culture supernatant was collected and the concentrations of the inflammatory factors were determined by ELISA. MiRNA complementary to Cx-43 was designed through the online tools.
Results: Cx-43 is upregulated in the treatment of gamma-radiation in astrocytes and gamma-radiation introduced the detrimental function of astrocytes: cell viability was reduced while the apoptotic cells were increased. Inflammatory factors like tumor necrosis factor alpha, interferon gamma, interleukin-6, interleukin 1-beta were dramatically up-regulated by the irradiation. MiR-374a rescued irradiation induced Cx-43 up-regulation of astrocytes and eliminated detrimental function triggered by gamma-radiation.
Conclusion: Cx-43 expression level may play an important role in the inflammation-related neural damage after irradiation-induced ischemic stroke.
[1]Peoples Hosp Pudong New Area, 490 South Chuanhuan Rd, Shanghai 201299, Peoples R China.
[2]Shanghai Univ Med & Hlth Sci, 279 ZhouZhu Rd, Shanghai 200120, Peoples R China.
[3]Nanchang Univ, Affiliated Hosp 1, Dept Neurosurg, Yong Wai Zheng St 17, Nanchang 330006, Jiangxi, Peoples R China.
(8.0+极速模式)