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Lobetyol activate MAPK pathways associated with G(1)/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo 期刊论文

编号：

d4943b25-059a-422e-86cc-1a9d73c569e9
作者：

Shen, Jie#^[1,6]Lu, XinGang#^[2]Du, WangChun(杜望春)^[3]Zhou, Jun^[4];Qiu, HongFu^[2];Chen, JingXian^[5];Shen, XiaoHeng^[5];Zhong, MingKang#*^[1]
语种：

英文
期刊：

BIOMEDICINE & PHARMACOTHERAPY ISSN：0753-3322 2016 年 81 卷 (120 - 127) ; JUL
收录：
关键词：

MKN45 Apoptosis Cell cycle arrest Lobetyol MAPK
摘要：

Aim: The agent lobetyol, which is isolated from Lobelia chinensis, was previously shown to be cytotoxicity againts several cancer cell lines in published report. Today, we perform a study in vitro and in vivo to analyze its anti-carcinoma effect in MKN45 cells and to explore the molecular mechanism.
Main methods: The growth inhibition of lobetyol on MKN45 cells was analyzed with MTT and flow cytometry. Hoechst 33342 staining and TUNEL cover glass staining were used to provide the visual evidence of apoptosis. Western blotting assay was performed to study the activation or blocking of related signaling pathways.
Key findings: Lobetyol induce apoptosis and cell cycle arrest in a time-and dose-dependent manner in MKN45 cells in our study. This process is mediated by the MAPK signaling pathways. This study confirmed the cytotoxicity of lobetyol in MKN45 cells and provided an insight into the molecular mechanism, which demonstrates the potential of lobetyol as an anti-tumor agent. (C) 2016 Elsevier Masson SAS. All rights reserved.
推荐引用方式
GB/T 7714：

Shen Jie,Lu XinGang,Du WangChun, et al. Lobetyol activate MAPK pathways associated with G(1)/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo [J].BIOMEDICINE & PHARMACOTHERAPY,2016,81:120-127.
APA：

Shen Jie,Lu XinGang,Du WangChun,Zhou Jun,&Zhong MingKang.(2016).Lobetyol activate MAPK pathways associated with G(1)/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo .BIOMEDICINE & PHARMACOTHERAPY,81:120-127.
MLA：

Shen Jie, et al. "Lobetyol activate MAPK pathways associated with G(1)/S cell cycle arrest and apoptosis in MKN45 cells in vitro and in vivo" .BIOMEDICINE & PHARMACOTHERAPY 81(2016):120-127.
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