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Administration of hypoxic pretreated adipose-derived mesenchymal stem cell exosomes promotes spinal cord repair after injury via delivery of circ-Astn1 and activation of autophagy 期刊论文

编号：

4B88FB3C3144EADDF43F5A08A791B038
作者：

Shao, Minghao#^[1,2]Jin, Mingming(靳明明)^[3]Lv, Feizhou*^[1]Ma, Xiaosheng*^[1]Wei, Zhu*^[1,2]
语种：

英文
期刊：

INTERNATIONAL IMMUNOPHARMACOLOGY ISSN：1567-5769 2025 年 152 卷 ; APR 16
收录：
关键词：

Adipose-derived mesenchymal stem cells Exosome Spinal cord injury Circ-Astn1 Microglia autophagy
摘要：

Background: The aim of this study was to investigate the role and mechanism of exosomes isolated from adiposederived mesenchymal stem cells (ADSCs) on spinal cord repair. Methods: High-throughput sequencing was used to investigate abnormal expression of circular RNA (circRNA) in ADSC exosomes pretreated under hypoxic conditions (HExos) and ADSCs exosomes under normal conditions (Exos). The abnormal expression of mRNA in spinal cord tissues was also analyzed using high-throughput sequencing. Bioinformatics and luciferase reporter analyses were used to clarify the relationship among circRNA, micro RNA (miRNA), and mRNA. BV2 cells were used to analyze apoptosis levels and inflammatory cytokine expression under oxygen-glucose deprivation (OGD) conditions by using immunofluorescence and enzyme-linked immunosorbent assay (ELISAs). An SCI mouse model was also constructed and the therapeutic effect of Exos was detected using immunohistochemistry and immunofluorescence. Results: High-throughput sequencing results showed that circ-Astn1 played a role in HExo-mediated spinal cord repair after SCI. Downregulation of circ-Astn1 decreased the therapeutic effect of HExos. We also found that Atg7 played a role in HExo-mediated spinal cord repair after SCI. Luciferase reporter analysis confirmed that both miR-138-5p and Atg7 were downstream targets of circ-Astn1. Downregulation of Atg7 or overexpression of miR138-5p reversed the protective effect of circ-Astn1 on BV2 cells after exposure to OGD conditions. In contrast, upregulation of circ-Astn1 increased the therapeutic effects of Exo-mediated spinal cord repair after SCI via autophagy activation. Conclusions: Taken together, the results indicate that ADSC-Exos containing circ-Astn1 promoted spinal cord repair after SCI by targeting the miR-138-5p/Atg7 pathway, which mediated autophagy.
推荐引用方式
GB/T 7714：

Shao Minghao,Jin Mingming,Lv Feizhou, et al. Administration of hypoxic pretreated adipose-derived mesenchymal stem cell exosomes promotes spinal cord repair after injury via delivery of circ-Astn1 and activation of autophagy [J].INTERNATIONAL IMMUNOPHARMACOLOGY,2025,152.
APA：

Shao Minghao,Jin Mingming,Lv Feizhou,Ma Xiaosheng,&Wei Zhu.(2025).Administration of hypoxic pretreated adipose-derived mesenchymal stem cell exosomes promotes spinal cord repair after injury via delivery of circ-Astn1 and activation of autophagy .INTERNATIONAL IMMUNOPHARMACOLOGY,152.
MLA：

Shao Minghao, et al. "Administration of hypoxic pretreated adipose-derived mesenchymal stem cell exosomes promotes spinal cord repair after injury via delivery of circ-Astn1 and activation of autophagy" .INTERNATIONAL IMMUNOPHARMACOLOGY 152(2025).
入库时间：

3/27/2025 9:14:52 PM
更新时间：

3/27/2025 9:14:52 PM

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